Hsc70 interacts with KCNA5 to promote the proliferation of granulosa cells in polycystic ovarian syndrome
نویسندگان
چکیده
Purpose: To investigate the function and interaction of heat-shock cognate protein 70 (Hsc70) potassium voltage-gated channel subfamily A member 5 (KCNA5) in polycystic ovary syndrome (PCOS).Methods: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) western blot were used to measure Hsc70 KCNA5 expression PCOS. The effect on granulosa cell apoptosis was determined by flow cytometry. Cell counting kit-8 (CCK-8) colony formation assay assess impact proliferation, while with evaluated Western blot, CCK-8, assay, respectively.Results: upregulated PCOS compared control (p < 0.05). In cells, overexpression promoted proliferation but inhibited 0.05), whereas knockdown suppressed Furthermore, downregulated 0.05) negatively correlated expression. also attenuated knockdown-mediated inhibition 0.05).Conclusion: promotes interacting KCNA5, further defining molecular mechanism behind
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ژورنال
عنوان ژورنال: Tropical Journal of Pharmaceutical Research
سال: 2022
ISSN: ['1596-5996', '1596-9827']
DOI: https://doi.org/10.4314/tjpr.v21i4.2